Gene expression data, sample check

2nd (Invited Participants) Challenge
1

Could you please check the 2020 gene expression data, pid 24 and 25? Could you please clarify the actual Day0 was happened prior to or post vaccine? I see that -13 and -6 are “Day0”, but the actual day “0” is still prior to vaccination or “day1”?

    specimen_id subject_id actual_day_relative_to_boost planned_day_relative_to_boost
114         181         24                          -13                             0
115         182         24                            0                             1
116         183         24                            2                             3
117         184         24                            6                             7
118         185         24                           13                            14
119         191         25                           -6                             0
120         192         25                            0                             1
121         193         25                            2                             3
122         194         25                            6                             7
123         195         25                           13                            14
2

For subject IDs 24 and 25, the actual_day_relative_to_boost = 0 occurred after the vaccination. The specimens from these two subjects were collected on the day of the tdap booster vaccination but after the vaccine was administered. That’s why these two specimens fall under day 1 of planned_day_relative_to_boost.